The activities of the liver an serum α-D-and β-D-glucosidase were studied for cholestasis induced by common bile duct ligation and chronic ethanol intoxication developed, or cholestasis after acute ethanol intoxication for manifestation of the
biochemical background of alcohol intoxication in hepatobiliary disease.
The groups that received common bile duct(CBD) ligation after being chronically intoxicated with ethanol showed a marked increase at the 7th and 14th day after the ligation in the liver lysosomal α-D-glucosidase activities. However, the
activities
showed a lower degree than groups of the CBD lgation.
The groups that received CBD ligation after being chronically intoxicated with ethanol showed a slight increase after the ligation in the liver lysosomal acid β-D-glucosidase and cytosolic broad specificity β-D-glucosidase activities, and the
same was
seen in the groups of CBD ligation.
At the 2.5th and 24th hours after the acute intoxication with ethanol with was done after 14 days of the CBD ligation, the rats showed a considerable increase in the liver lysosomal α-D-glucosidase, lysosomal acid β-D-glucosidase and cytosolic
broad
specitivity β-D-glucosidase activities, and the same was seen in the group sacrificed on the 14th day after the CBD ligation.
The groups that received CBD ligation after being chronically intoxicated with ethanol showed a marked increase after the ligation in the serum lysosomal α-D-glucosidase and broad specificity β-D-glucosidase activities. However, the activities
showed
a far higher degree than groups of the CBD ligation. For the groups of acute intoxication with ethanol done after 14 days of the CBD ligation, the serum broad specificity β-D-glucosidase activities increased markadly, but the activities showed a
higher
degree than the group with the 14th day after the CBD ligation.
In summary, especially, when the acute and chronic ethanol intoxication with cholestasis occurred, the serum lysosomal α-D-glucosidase and broad specificity β-D-glucosidase are higher than in cholestasis because of increased liver cell damage,
which
causes the enzyme to leak into the blood in great quantity. Accordingly, these results will be the data supporting the alcoholic drink is enzymologically harmful in hepatobiliary diseases.
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